Analytical method development and validation analysis for quantitative assessment of cypermethrin by HPLC procedure

The precise, systematic, definite, particular, linear, exact and robust scientific method was developed and validated for the assay of Cypermethrin in Cypermethrin 25% EC Insecticide for Injection, USP 500 mg/vial. Presently utilized Cypermethrin 25 % EC Insecticide as a working standard having limit was 95% for method development and validation. The quantitative determination bring to accomplished by HPLC- Waters - Alliance 510 system equipped with UV/PDA detector. Methanol, Acetonitrile and water in the ratio (60:20:20 v/v/v) used as mobile phase and flow rate 1.0 ml / min. with 20 minutes run time. The detection was carried at 225 nm with Nucleosil C18 column (250 mm × 4.6 mm x 10 µm) and ambient column temperature was maintained. In this connection, method uses the 20 μl injection volume and diluent as a blank solution. The linearity of this method was found to be linear in the range of 50% to 150% of the working concentration and the range for the analytical method is 25 ppm to 75 ppm. The accuracy and precision of the method were within acceptable. The present developed HPLC method is found to be suitable. The analytical solution was found to be stable up to 48 Hrs at room temperature.