Design of primers for evaluation of some gram-negative bacteria isolated from intensive care unit patients in Wasit province, Iraq

Intensive care unit (ICU) patients frequently have consequences from healthcare-associated infections (HAIs), which include bacteremia, pneumonia, urinary tract, skin, or soft tissue infections. . In this study, a total of 100 clinical specimens (urine, sputum and pus) were collected from patients admitted in the ICU. Results showed eighty two were positive growth culture ‎and admitted to the intensive care unit distributed 1 (1.2%) Klebsiella oxytoca, 5(6.25%)‎Proteus mirabilis, 2 (2.4%)‎ Acinetobacter baumannii , 1 (1.2%)‎ Serratia marcescens and 1 (1.2%)‎ Burkholderia cepacia group bacteria . The results of this study showed that the diagnostic test for 16S rRNA ‎bacteria (Proteus mirabilis), which numbered 5 (100%), bacteria ‎‎(Acinetobacter baumannii), which numbered 2 (100%), bacteria ‎‎(Burkholderia cepacia ), which numbered 1 (100%), bacteria ‎‎(Serratia marcescens ), which numbered 1 (100%), and Bacteria ‎‎(klebsiella oxytoca ), which numbered 1 (100%) after completing the ‎phenotypic and biochemical diagnosis diagnostic test (16S rRNA).