Enzyme activities in the spent mushroom substrate from Pleurotus ostreatus

Spent mushroom substrate (SMS) from Pleurotus ostreatus is residue of mushroom cultivation that is known to have lignocellulolytic enzyme activity in it. Enzyme activities provides information on evidence of fungi converting complex substrates to simple ones as nutrients for them. In this study, the enzyme was found cellulase, lignin peroxidase, and protease. The samples used consisted of three samples of SMS were collected from Padang Panjang (sample 1), Padang (sample 2), Payakumbuh (sample 3). Research was conducted to determine and compare the activity of cellulase, protease, and lignin peroxidase enzymes were evaluated as potential source of enzyme and also to determine the levels of reducing sugar, ph, and C/N ratio. Maximum activities in the SMS were 0.179 U/g cellulase, 3584.229 U/g LiP, and 1400 U/g. The value range 16,459 mg/ml to 19,845 mg/ml of reducing sugar, 5.78 to 8.08 of pH and 24.053% to 81.345% of C/N ratio. The result reveal that SMS from P. ostreatus was a good and could be a potential source of enzymes for alternative in the circular economy concept.