Isolation and characterization of the bioactive components in Tetracarpidium conophorum as dissolvent in methanol

This study evaluate  bioactive compounds present in the methanol  extracts of raw Tetracarpidium conophorum (African walnut) with their molecular formulae, molecular weights, Retention Times (RT) and Peak areas (%), are shown in Table 1. The GC-MS chromatograms detected 13 peaks of bioactive compounds for extract as shown in Figure 2.The height of the peak indicates the relative concentration of the components present in the extracts. These compounds mainly comprised esters, alcohols, hydrocarbons and ketones. The major chemical constituents identified in the raw extract was 9,12,15-octadecatrienoic acid, (Z, Z,Z)- (67.61%), other compounds identified were n-hexadecanoic acid (4.92%); 9,12-octadecadienoic acid, methyl ester (0.27%); 9,12,15-octadecatrienoic acid, methyl ester, (Z,Z,Z)- (1.38%); heptadecanoic acid, 16-methyl-, methyl ester (0.10%); 9,12,15-octadecatrienoic acid, ethyl ester, (Z,Z,Z)- (17.15%); ethyl 9,12,15- octadecatrienoate (5.21%); 9,12,15-octadecatrien1-ol, (Z,Z,Z)- (2.85%); 9,12-octadecadienoic acid (Z,Z)- (9.55%); methyl 8,11,14 heptadecatrienoate (4.35%); octadecanoic acid (3.87%); bis(2-ethylhexyl) phthalate (0.11%) and squalene (0.23%).