The difference of fungal growth Rhizopus sp. in SDA and PDA Media from toe nail samples of construction workers

Onychomycosis is a fungal infection caused by dermatophyta and non-dermatophyte groups which infect the toenails or fingernails. Onychomycosis infection can lead to the damage of the nails which causes the nail thickness, brittle, and easily destroyed. Rhizopus sp. is considered to be one of the fungal species in the non-dermatophyte fungal infection group in the disease Onychomycosis. This study aims to determine the differences in the growth of non-dermatophyte fungi that cause onychomycosis infections on SDA and PDA media in samples of toenails from construction workers in Lamongan Regency. The research method used is descriptive, and the sampling technique used is random sampling. The sample size examined was 15 construction workers in duplicate, 15 planted in PDA media and 15 planted in SDA media. The material for this examination is scrapings from the toenails of construction workers which are planted directly on SDA and PDA media, then followed by macroscopic and microscopic examination. Based on macroscopsic examination, it was found that the colonies were white to grayish white, had a cotton-like surface and were spreading. Based on microscopic examination using the addition of 1 drop of LCB dye reagent, fungal elements were obtained which indicated the non-dermatophyte fungus Rhizopus sp. namely there are stolon, rhizoid, sporangiospore, sporangium and apoplysis. From the results of this research it can be concluded that there is growth of the fungus Rhizopus sp. non-dermatophyte group on PDA media and SDA media, there was no difference in the growth of non-dermatophyte fungi that cause onychomycosis in the toenails of construction workers in Lamongan Regency on PDA media and SDA media and there was a difference in the growth of the fungus Rhizopus sp. Non-dermatophyte groups causing onychomycosis based on age.