RP UFLC method for estimation of valsartan chemometricaly

A simple, sensitive, rapid, precise and economical reverse phase HPLC method has been developed for the estimation of valsartan from pharmaceutical dosage forms. The method was carried out  using Chromatographic conditions were established by employing a C18 analytical phenomenal (Kinetex) column (5micron -C18, 250x4.6mm) with a mobile phase of phosphate buffer (PH 3.5) and acetonitrile in a 60:40 ratio. The sample was injected at a volume of 20 microliters, and the mobile phase was degassed using a Sonica ultrasonic sonicator before being pumped into the HPLC system. The flow rate was set at 1 ml per minute, and the wave length 273 nm was chosen for detection. The column temperature was kept at 25oC respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation and solution stability. The proposed method can be used for the estimation of these drugs in dosage forms.