The effect of Abelmoschus esculentus pods ethanol extract on mice (Mus musculus) preantral follicle, antral follicle, and corpus luteum numbers exposed to carbon black

Introduction: This study aims to determine the preventive effect of okra (Abelmoschus esculentus) pods ethanol extract on the mice (Mus musculus) preantral follicle, antral follicle, and corpus luteum numbers exposed to carbon black.
Material and Methods: This study used 25 mice which were divided into 5 groups. The negative control group (C-) and the positive control group (C+) were given Na-CMC 0.5% solution orally, while the treatments groups T1, T2, and T3 were pretreated with okra pods ethanol extract orally with a dose of 4 mg/20gBW, 8mg/20gBW and 16 mg/20gBW. Later the C+, T1, T2, and T3 were exposed to 1064 mg/m3 carbon black for 6 hours/day for 30 days.
Results: The results of the study showed that there was a significant difference between control group C- (18.20c±2.28; 11.20d±0.83), C+ (8.60a±1.14; 5.00a±1.00), treatment group T1 (12.60b±2.70; 8.00b±1.22),  T2  (15.20bc±4.76;  8.80bc±2.04)  and  T3 (17.80c±2.77;  9.80cd±0.83) (p<0.05) in ovarian preantral and antral follicle numbers, while there was not shown any significant difference between the corpus luteum numbers (p>0.05) in all treatment groups. 
Conclusion: It can be concluded that okra pods (Abelmoschus esculentus) ethanol extract can maintain the preantral and antral follicle numbers in mice exposed to carbon black.