Standardization of Anda Neer prepared as per Siddha Literature

Siddha drugs are natural products obtained from herbal, mineral and animal kingdom. Most of the Siddha medicines are effective but they have not been standardized yet. So, there be in a need to progress a standardization technique. Anda Neer is a classical Siddha preparation for curing redness of eye mentioned in the textbook of Siddhar Aruvai Maruthuvam written by Dr. K. S. Uthamarayan.. The aim of this study to estimate quality of Anda Neer by conducting physico-chemical analysis, and other analytical techniques. This study was done based on Pharmacopoeial laboratory for Indian Medicine guidelines. Other analytical technique like High performance thin layer chromatography was done through CAMAG software. The physical parameters like state, odour, touch, flow Property and appearance/colour revealed that it was liquid, characteristic odour, non-greasy to touch, free flowing, colourless and transparent. So the drug AN is good in nature and safe to use. The pH value of AN was 7 which indicates that the drug is neutral in nature. A more acidic or alkaline pH value induces lacrimation, ocular pain, and discomfort. In the clarity test observation it was found as clear solution with no major visible particles. HPTLC finger print analysis of the extracts showed the presence of possible number of components. The test drug is devoid of microbial contamination, pesticide residues and aflatoxin assay were below quantification limit. From the result we infer that the drug is of standard quality and shall be used as reference in pharmacopoeia standards.